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Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
neoglycoconjugates

Previously, we have developed a versatile and very convenient approach to the synthesis of neoglycoconjugates (see review). The method is based on attachment of different aminoligands, both carbohydrate and non-carbohydrate ones, labels and effectors, to fully activated polymer.

Diversity of PAA-based carbohydrate probes.

Recently, the approach was greatly extended.

  • The range of oligosaccharides subjected to attachment was considerably increased paying particular attention to sialylated and sulfated derivatives. Our experience shows that there are no limitations in applicability of these acid oligosaccharides.

  • High molecular weight (~1-2 mln Da) PAA-conjugates were synthesized. The size of molecules is about 50 nm. The probes are capable to multipoint binding with antibodies, viruses and all lectins. In a number of cases the binding was 2-3 orders of magnitude higher than with common 30 kDa PAA-conjugates, as it was demonstrated for interaction of sialylated probes with influenza viruses and with siglec-2.

  • Affinity gels for EF separation of lectins and anti-carbohydrate antibodies been have developed. An additional layer is added to common EF-gel whereto a derivative Sug-PAA-allyl is copolymerized. Due to the latter, either the studied protein is fixed in affine part of the gel related, whereas all the rest proteins are separated like in case of common EF.

  • A convenient method for neoglycoconjugates immobilization on any surface (plastic, glass, metal, diamond etc.) has been proposed. This approach allows to set various parameters, e.g. clusterness of carbohydrate ligands and density on the surface.

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