1. The nature of so-called Tk antigen has been studied. This antigen is related to both blood group specific and tumor-associated antigens. Tk appears on erythrocytes after their treatment with endogalactosidase unmasking the terminal GlcNAc residues. Anti-Tk monoclonal antibodies bound specifically tumors including gastro-intestinal ones but did not bind to membrane antigens of normal cells, thus interacting only with intracellular components of normal cells. Using synthetic oligosaccharides, specificity of these antibodies was determined. The supposition that they bind to the branched motif
2. Supposing that a tumor is an inflammation site, selectin ligand SiaLex (see ‘Selectins’) was used as specific vector (lipophilic form of the tetrasaccharide) for delivery of cytotoxic liposomes to tumor . Indeed, such liposomes caused drastic increase in life time of mice with grafted tumors, even in case of single preparation administration.
3. The role of carbohydrate chains in phagocytosis of apoptotic bodies obtained from melanoma cell line has been studied on a model where macrophage cell line was used. It was shown that termination