Polyacrylamide-Based Glycoconjugates As Tools in Glycobiology.
Glycoconjugate Journal, 15 (5), pp. 431 - 446, (1998)
Shemyakin Institute of Bioorganic Chemistry, Russian Academy of Sciences, ul. Miklukho-Maklaya 16/10, Moscow GSP-7, 117871, Russia
This review describes the synthesis, physicochemical characteristics and application for studying carbohydrate-binding proteins of polyacrylamide (PAA) type neoglycoconjugates. An approach to the synthesis of conjugates based on the interaction of activated polyacrylic acid with ω-aminoalkyl glycosides has been developed. Both the molecules of Glyc-PAA and the conjugates bearing various labels and effectors, as well as sorbents, and glycosurfaces can be designed using this method. Examples of the application of the conjugates as tools for the study of lectins, antibodies, and glycosyltransferases in glycobiology, cytochemistry and histochemistry are described along with the prospects of the further development of the presented approach in glycotechnology and medicine.
Keywords: antibodies, assays, carbohydrates, cytochemistry, diagnostics, glycoconjugates, glycosyltransferases, histochemistry, immunoassay, lectins, polyacrylamide