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Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
Abstract
[print version]

S.D.Shiyan, V.S.Zueva, V.V.Nasonov, L.S.Zhigis, N.C.Coi, N.V.Bovin
Sialylation of N-Carbohydrate Chains of Glycoproteins with Trans-Sialidase from Trypanosoma cruzi. [Article in Russian]
Russ. J. Bioorgan. Chem., 30 (4), pp. 358 - 366, (2004)

α2,3-Sialylation of the lactosamine type N-glycans with trans-sialidase from Trypanosoma cruzi is reported. Trans-sialidase (160 kDa, pI 5.355.65) and its catalytic fragment (70 kDa, pI 6.06.3) were isolated from T. cruzi cells and immobilized on ConA-Sepharose. The resulting preparation retained its activity for several months and was repeatedly used for obtaining mono-, di-, tri-, and tetrasialylated 7-amino-4-metylcoumarin-labeled oligosaccharides with various numbers of antennas and for α2,3-sialylation of glycans within glycoproteins and neoglycoconjugates

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